In a second step, the cumulative score in each group was correlated to the final histological diagnosis in order to establish a score range for normal and hyperplasia. Please help improve this section by adding citations to reliable sources. No use, distribution or reproduction is permitted which does not comply with these terms.
The sample of tissue is immersed in fixative solution of volume at a minimum of 20 times greater than the volume of the tissue to be fixed. Rats were subcutaneously injected with testosterone propionate in corn oil 0.
The stained sections were dehydrated in the graded ethanol series and xylene and then mounted with Canada balsam neutral mounting medium.
Allium cepa chromosome aberration and micronucleus test applied to study genotoxicity of extracts from pesticide-treated vegetables and grades. Numerous studies have revealed that garlic extract, volatile oil, allicin, and mercaptan sulfur compounds have strong antibacterial, antifungal, and antiviral activities Ankri and Mirelman, ; Avato et al.
Histopathology and Histoscore A part of the ventral lobes was separated and fixed overnight in Stieve's solution. Environmental Monitoring and Assessment, Using a larger sample means it takes longer for the fixative to reach the deeper tissue.
The fixative must diffuse through the tissue to fix, so tissue size and density, as well as type of fixative must be considered. Even the most careful fixation does alter the sample and introduce artifacts that can interfere with interpretation of cellular ultrastructure.
Following ligation of the spermatic cord and vessels, testes and epididymis were removed.
The scoring system was presented in arbitrary units to make a better evaluation. The use of allelopathic crops to control weeds, alleviate obstacles to continuous cropping or promote crop growth in agriculture is currently being tested Cheema and Khaliq, ; Cheema et al.
Report and Opinion, 3 1: Ten to fifteen root meristems obtained from three to four bulbs per treatment were analysed. However, the fresh weights of tomato hypocotyls treated with DADS were not affected, except at concentrations of 2.
The capability of the extracts to scavenge the DPPH radical was calculated using the following equation: For each gene analysis, three biological replicates were performed.
Furthermore, it is known that mutagens significantly affect the sex organs, the most sensitive elements of a living organism and may seriously alter their fertility Kaymak, An evaluation of genotoxicity Cresencio C. The method of Allium anaphase-telophase chromosome aberration assay.
Similar observations have also been reported by others, in the other systems Amer and Ali,; Chauhan et al. Cytological effects of pesticides. Tissue is then sectioned in a freezing microtome or cryostat and sections are fixed in one of the following fixatives: Mitotic disturbances induced by low doses of inorganic lead.
Table 1 Sequences of oligonucleotides used as primers. This study was aimed to detect the effect of copper wastes on cell division and somatic chromosomes of Allium cepa.
Chromosomal and nuclear alterations in root tip cells of Allium cepa L. It also recorded the highest dividing cells and fewer in a number of aberrant cells.
The tissue sections were heated in Ventana buffer CC1 pH 6 to facilitate antigen retrieval and treated with H2O2 to eliminate endogenous peroxidase.
The germinated seeds were placed between the bottom of the dish and the filter paper. Furthermore, plant roots are extremely useful in biological testing because root tips are the first to be exposed to toxicants dispersed in soil or in water Fiskesjo, The anticancer properties of DADS have been studied in experimental animals and various types of cancer cells, but to date, little is known about its mode of action as.
After 48 hr, 2 onions out of 12 with most poorly growing roots were removed, distal 2 mm of five roots was cut off from five bulbs and were fixed in acetoalcohol ( v/v) for the chromosomal study.
The fixation was done at a fixed time in all experiments. After 96 hr, length of 05 root bundles (all the roots of a bulb) from each onion were measured. Composition of Fixatives: 1-Formalin Solution (10%, unbuffered): Formaldehyde (%) - 10 ml Distilled water - 90 ml Mix wellFormalin Solution (10%, buffered neutral): Formaldehyde (%) - ml Distilled water - ml NaH2PO4 - g Na2HPO4 (anhydrous) - g Mix to dissolve.
Start studying Urinalysis. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Search. B. Quantitative urine assays require a timed urine collection to mitigate the effects of the following: 1.
Diurnal or circadian variation in excretion of many substances May need cytological evaluation to identify source. Cytological And Histochemical Studies On Rat Liver And Pancreas During onions and garlic are good for maintaining blood glucose levels coming to this fact. Antioxidants can exert beneficial effects on both liver and pancreatic B-cell function in diabetes (Calabrese, ).
Thus a. Despite its known effects, lots are still using formalin or formaldehyde in their goods preservation.! Scandals have broken in both the Indonesia food scare and Vietnam food scare regarding the addition of formaldehyde to foods to extend shelf life.Download